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HelpTest Code TRAMS Tramadol and Metabolite, Serum
Specimen Required
Supplies: Sarstedt Aliquot Tube, 5 mL (T914)
Collection Container/Tube: Red top (Serum gel/SST are not acceptable)
Submission Container/Tube: Plastic vial
Specimen Volume: 1 mL
Collection Instructions: Within 2 hours of collection, centrifuge and aliquot serum into a plastic vial.
Useful For
This test is useful to help determine compliance and/or to monitor adverse drug reactions or efficacy
Method Name
Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS)
Reporting Name
Tramadol and metabolite, SSpecimen Type
Serum RedSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time |
|---|---|---|
| Serum Red | Refrigerated (preferred) | 28 days |
| Ambient | 28 days | |
| Frozen | 28 days |
Reference Values
Tramadol:
Peak plasma levels following a single 100 mg oral dose: 230-380 ng/mL
Steady-state plasma levels following a 100 mg 4 times daily regimen: 420-770 ng/mL
O-desmethyltramadol:
Peak plasma concentration following a single 100 mg oral dose: 35-75 ng/mL
Steady-state plasma concentration following a 100 mg 4 times daily regimen: 80-140 ng/mL
Cutoff concentrations by liquid chromatography tandem mass spectrometry:
Tramadol: 10 ng/mL
O-desmethyltramadol: 5.0 ng/mL
Day(s) Performed
Wednesday
Report Available
3 to 9 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
G0480
80373 (if appropriate for select payers)
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| TRAMS | Tramadol and metabolite, S | 111526-0 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 623268 | Tramadol | 12437-0 |
| 623269 | O-desmethyltramadol | 82974-7 |
Forms
If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.