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Test Code CLDPCR Clostridium difficile - Molecular Testing

Additional Codes

LAB5400

Methodology

PCR testing for Clostridium difficile and presence of NAP1 strain

Performing Laboratory

St. Mary's Regional Medical Center-Microbiology

Specimen Requirements

Orders for specimens x3 are not accepted.

PCR testing is highly specific and sensitive and only one sample is required.

Only one specimen per 7 days is accepted if the previous result was NEGATIVE.

Only one specimen per 10 days is accepted if the previous result was POSITIVE.

 

1. Submit 1 mL of liquid  stool in a clean, dry container.Stool must be a 7 on the Bristol scale. 
2. Label container with patient’s name, date of birth, and date of collection,

3. Forward promptly at ambient temperature. If there is a delay in transport, send specimen refrigerated.

4. Specimens must be transported in biohazard bags to prevent cross-contamination.

5. Causes for rejection:

- Inadequately labeled specimen

- Improper container

- Insufficient specimen volume

- Unacceptable consistency of stool

- No more than one specimen within seven days for patients with negative C. difficile results.

- No more than one specimen within 10 days for patients with positive C. difficile results.

- Test must not be used for test of cure.

Reference Values

Negative:  Specimen negative for toxigenic C. difficile by DNA amplification. A negative result indicates no C. difficile DNA present.

 

NAP1 Presumptive Negative: NAP1 strain presumptively not present.

 

 

Day(s) Test Set Up

Monday through Sunday, first shift only.